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keap1  (Sino Biological)


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    Sino Biological keap1
    Keap1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/keap1/product/Sino Biological
    Average 93 stars, based on 18 article reviews
    keap1 - by Bioz Stars, 2026-06
    93/100 stars

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    Oxidative stress response and the KEAP1–NRF2 pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Journal: Frontiers in Physiology

    Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments

    doi: 10.3389/fphys.2026.1688832

    Figure Lengend Snippet: Oxidative stress response and the KEAP1–NRF2 pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Article Snippet: Serum KEAP1 and Nrf2 levels were measured using a human KEAP1 enzyme-linked immunosorbent assay (ELISA) kit (EH4240, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China) and a human Nrf2 ELISA kit (abs551899, Absin Bioscience Inc., Shanghai, China) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control

    KEAP1–NRF2-mediated oxidative stress participates in the occurrence of COPD combined with CAS in mice. (A) PFT for FRC, RI, Cdyn, and MV in mice. (B) The histopathological changes of lung tissues observed using H&E staining. (C) Serum lipid levels in mice measured via ELISA. (D) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of mice determined via RT-qPCR. (E) Levels of ROS and MDA, and activities of SOD and catalase in the lung tissue of mice measured using kits. (F) Representative Western blotting of KEAP1, NRF2, NQO1, and HO-1 proteins in the lung tissue of mice and the quantification data. n = 6 mice for each treatment. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests for multiple comparisons. COPD, chronic obstructive pulmonary disease; CAS, coronary atherosclerosis; PFT, pulmonary function test; FRC, functional residual capacity; RI, resistance index; Cdyn, dynamic compliance; MV, minute ventilation; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Journal: Frontiers in Physiology

    Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments

    doi: 10.3389/fphys.2026.1688832

    Figure Lengend Snippet: KEAP1–NRF2-mediated oxidative stress participates in the occurrence of COPD combined with CAS in mice. (A) PFT for FRC, RI, Cdyn, and MV in mice. (B) The histopathological changes of lung tissues observed using H&E staining. (C) Serum lipid levels in mice measured via ELISA. (D) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of mice determined via RT-qPCR. (E) Levels of ROS and MDA, and activities of SOD and catalase in the lung tissue of mice measured using kits. (F) Representative Western blotting of KEAP1, NRF2, NQO1, and HO-1 proteins in the lung tissue of mice and the quantification data. n = 6 mice for each treatment. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests for multiple comparisons. COPD, chronic obstructive pulmonary disease; CAS, coronary atherosclerosis; PFT, pulmonary function test; FRC, functional residual capacity; RI, resistance index; Cdyn, dynamic compliance; MV, minute ventilation; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Article Snippet: Serum KEAP1 and Nrf2 levels were measured using a human KEAP1 enzyme-linked immunosorbent assay (ELISA) kit (EH4240, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China) and a human Nrf2 ELISA kit (abs551899, Absin Bioscience Inc., Shanghai, China) according to the manufacturer’s instructions.

    Techniques: Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Functional Assay